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cell lines hek 293 atcc crl 1573 vero e6 atcc crl  (ATCC)


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    Structured Review

    ATCC cell lines hek 293 atcc crl 1573 vero e6 atcc crl
    Cell Lines Hek 293 Atcc Crl 1573 Vero E6 Atcc Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 22465 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+hek+293+atcc+crl+1573+vero+e6+atcc+crl/293/pm37068395-239-186-189
    Average 99 stars, based on 22465 article reviews
    cell lines hek 293 atcc crl 1573 vero e6 atcc crl - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Virus:

    Article Title: Pre-existing immunity modulates responses to mRNA boosters.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD8, clone 53-6.7 in PerCPcy5.5 BD Pharmingen Cat# 551162, RRID:AB_394081 aCD44, clone IM7 in Pacific Blue Biolegend Cat# 103020, RRID:AB_493683 aIFNy, clone XMG1.2 in APC BD Pharmingen Cat# 554413, RRID:AB_398551 aCD4, clone RM4-5 FITC eBioscience Cat# 11-0042-82, RRID:AB_464896 Goat a mouse IgG-biotin, clone RM4-5 FITC Invitrogen Cat# SA5-10239, RRID:AB_2810197 Streptavidin (SA)-APC Invitrogen S868 biotin anti-mouse IgG1[a] BD-Pharmingen Cat# 553500, RRID:AB_394885 biotin anti-mouse IgG1[b] BD-Pharmingen Cat# 553533, RRID:AB_394903 anti-SARS-CoV-2 S protein (RBD epitope B), clone SARS2-34 BioXCell Cat# BE0359, RRID:AB_2894778 anti-SARS-CoV-2 S protein (RBD epitope A), clone SARS2-01 BioXCell Cat# BE0357, RRID:AB_2894776 Bacterial and virus strains Ad5-SARS CoV2 S University of Iowa Vector Core, custom made N/A SARS-Related Coronavirus 2, Isolate USA-WA1/2020, BEI resources NR-52281 Chemicals, peptides, and recombinant proteins SARS CoV-2 S protein overlapping peptide pools (181 peptides) BEI resources NR-52402 SARS CoV-2 S protein for ELISA Made by the Northwestern University recombinant protein production core using a SARS CoV-2 S protein gene provided by BEI (NR-52394, see above) N/A Critical commercial assays Quick RNA 96 kit (RNA extraction) Zymo Research R1052 Taqman RNA-to-CT step kit ThermoFisher 4392653 Experimental models: Cell lines HEK-293 ATCC CRL-1573 Vero-E6 ATCC CRL-1586 Experimental models: Organisms/strains K18-hACE2 mice Jackson Laboratories 034060 Recombinant DNA Plasmid containing SARS CoV-2 spike (S) gene BEI resources NR-52394 Software and algorithms GraphPad Prism software version 9.1.0 https://www.graphpad.com/ scientific-software/prism/ N/A FlowJo version 10.7.2 https://www.flowjo.com N/A Other KbVL8 tetramer (APC) Provided by the NIH tetramer core at Emory University N/A Forward primer, 50 GACCCCAAAATCAGCGAAAT-30 Integrated DNA Technologies 10006713 (Continued on next page) Cell Reports 42, 112167, March 28, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Reverse primer, 50 TCTGTTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 Probe, 50 TCTGGTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 SARS CoV-2 copy number control BEI resources NR-52358 Live dead dye for flow cytometry Invitrogen L34976 Cytofix/cytoperm (fixing agent for flow cytometry) BD Biosciences 554722 Phosphate buffer saline (PBS) Gibco 14190-144 Golgi Plug BD Biosciences 555029 Golgi Stop BD Biosciences 554724 DMEM Gibco 11965-092 Fetal bovine serum Sigma F0926-500

    Recombinant:

    Article Title: Pre-existing immunity modulates responses to mRNA boosters.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD8, clone 53-6.7 in PerCPcy5.5 BD Pharmingen Cat# 551162, RRID:AB_394081 aCD44, clone IM7 in Pacific Blue Biolegend Cat# 103020, RRID:AB_493683 aIFNy, clone XMG1.2 in APC BD Pharmingen Cat# 554413, RRID:AB_398551 aCD4, clone RM4-5 FITC eBioscience Cat# 11-0042-82, RRID:AB_464896 Goat a mouse IgG-biotin, clone RM4-5 FITC Invitrogen Cat# SA5-10239, RRID:AB_2810197 Streptavidin (SA)-APC Invitrogen S868 biotin anti-mouse IgG1[a] BD-Pharmingen Cat# 553500, RRID:AB_394885 biotin anti-mouse IgG1[b] BD-Pharmingen Cat# 553533, RRID:AB_394903 anti-SARS-CoV-2 S protein (RBD epitope B), clone SARS2-34 BioXCell Cat# BE0359, RRID:AB_2894778 anti-SARS-CoV-2 S protein (RBD epitope A), clone SARS2-01 BioXCell Cat# BE0357, RRID:AB_2894776 Bacterial and virus strains Ad5-SARS CoV2 S University of Iowa Vector Core, custom made N/A SARS-Related Coronavirus 2, Isolate USA-WA1/2020, BEI resources NR-52281 Chemicals, peptides, and recombinant proteins SARS CoV-2 S protein overlapping peptide pools (181 peptides) BEI resources NR-52402 SARS CoV-2 S protein for ELISA Made by the Northwestern University recombinant protein production core using a SARS CoV-2 S protein gene provided by BEI (NR-52394, see above) N/A Critical commercial assays Quick RNA 96 kit (RNA extraction) Zymo Research R1052 Taqman RNA-to-CT step kit ThermoFisher 4392653 Experimental models: Cell lines HEK-293 ATCC CRL-1573 Vero-E6 ATCC CRL-1586 Experimental models: Organisms/strains K18-hACE2 mice Jackson Laboratories 034060 Recombinant DNA Plasmid containing SARS CoV-2 spike (S) gene BEI resources NR-52394 Software and algorithms GraphPad Prism software version 9.1.0 https://www.graphpad.com/ scientific-software/prism/ N/A FlowJo version 10.7.2 https://www.flowjo.com N/A Other KbVL8 tetramer (APC) Provided by the NIH tetramer core at Emory University N/A Forward primer, 50 GACCCCAAAATCAGCGAAAT-30 Integrated DNA Technologies 10006713 (Continued on next page) Cell Reports 42, 112167, March 28, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Reverse primer, 50 TCTGTTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 Probe, 50 TCTGGTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 SARS CoV-2 copy number control BEI resources NR-52358 Live dead dye for flow cytometry Invitrogen L34976 Cytofix/cytoperm (fixing agent for flow cytometry) BD Biosciences 554722 Phosphate buffer saline (PBS) Gibco 14190-144 Golgi Plug BD Biosciences 555029 Golgi Stop BD Biosciences 554724 DMEM Gibco 11965-092 Fetal bovine serum Sigma F0926-500

    Enzyme-linked Immunosorbent Assay:

    Article Title: Pre-existing immunity modulates responses to mRNA boosters.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD8, clone 53-6.7 in PerCPcy5.5 BD Pharmingen Cat# 551162, RRID:AB_394081 aCD44, clone IM7 in Pacific Blue Biolegend Cat# 103020, RRID:AB_493683 aIFNy, clone XMG1.2 in APC BD Pharmingen Cat# 554413, RRID:AB_398551 aCD4, clone RM4-5 FITC eBioscience Cat# 11-0042-82, RRID:AB_464896 Goat a mouse IgG-biotin, clone RM4-5 FITC Invitrogen Cat# SA5-10239, RRID:AB_2810197 Streptavidin (SA)-APC Invitrogen S868 biotin anti-mouse IgG1[a] BD-Pharmingen Cat# 553500, RRID:AB_394885 biotin anti-mouse IgG1[b] BD-Pharmingen Cat# 553533, RRID:AB_394903 anti-SARS-CoV-2 S protein (RBD epitope B), clone SARS2-34 BioXCell Cat# BE0359, RRID:AB_2894778 anti-SARS-CoV-2 S protein (RBD epitope A), clone SARS2-01 BioXCell Cat# BE0357, RRID:AB_2894776 Bacterial and virus strains Ad5-SARS CoV2 S University of Iowa Vector Core, custom made N/A SARS-Related Coronavirus 2, Isolate USA-WA1/2020, BEI resources NR-52281 Chemicals, peptides, and recombinant proteins SARS CoV-2 S protein overlapping peptide pools (181 peptides) BEI resources NR-52402 SARS CoV-2 S protein for ELISA Made by the Northwestern University recombinant protein production core using a SARS CoV-2 S protein gene provided by BEI (NR-52394, see above) N/A Critical commercial assays Quick RNA 96 kit (RNA extraction) Zymo Research R1052 Taqman RNA-to-CT step kit ThermoFisher 4392653 Experimental models: Cell lines HEK-293 ATCC CRL-1573 Vero-E6 ATCC CRL-1586 Experimental models: Organisms/strains K18-hACE2 mice Jackson Laboratories 034060 Recombinant DNA Plasmid containing SARS CoV-2 spike (S) gene BEI resources NR-52394 Software and algorithms GraphPad Prism software version 9.1.0 https://www.graphpad.com/ scientific-software/prism/ N/A FlowJo version 10.7.2 https://www.flowjo.com N/A Other KbVL8 tetramer (APC) Provided by the NIH tetramer core at Emory University N/A Forward primer, 50 GACCCCAAAATCAGCGAAAT-30 Integrated DNA Technologies 10006713 (Continued on next page) Cell Reports 42, 112167, March 28, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Reverse primer, 50 TCTGTTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 Probe, 50 TCTGGTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 SARS CoV-2 copy number control BEI resources NR-52358 Live dead dye for flow cytometry Invitrogen L34976 Cytofix/cytoperm (fixing agent for flow cytometry) BD Biosciences 554722 Phosphate buffer saline (PBS) Gibco 14190-144 Golgi Plug BD Biosciences 555029 Golgi Stop BD Biosciences 554724 DMEM Gibco 11965-092 Fetal bovine serum Sigma F0926-500

    RNA Extraction:

    Article Title: Pre-existing immunity modulates responses to mRNA boosters.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD8, clone 53-6.7 in PerCPcy5.5 BD Pharmingen Cat# 551162, RRID:AB_394081 aCD44, clone IM7 in Pacific Blue Biolegend Cat# 103020, RRID:AB_493683 aIFNy, clone XMG1.2 in APC BD Pharmingen Cat# 554413, RRID:AB_398551 aCD4, clone RM4-5 FITC eBioscience Cat# 11-0042-82, RRID:AB_464896 Goat a mouse IgG-biotin, clone RM4-5 FITC Invitrogen Cat# SA5-10239, RRID:AB_2810197 Streptavidin (SA)-APC Invitrogen S868 biotin anti-mouse IgG1[a] BD-Pharmingen Cat# 553500, RRID:AB_394885 biotin anti-mouse IgG1[b] BD-Pharmingen Cat# 553533, RRID:AB_394903 anti-SARS-CoV-2 S protein (RBD epitope B), clone SARS2-34 BioXCell Cat# BE0359, RRID:AB_2894778 anti-SARS-CoV-2 S protein (RBD epitope A), clone SARS2-01 BioXCell Cat# BE0357, RRID:AB_2894776 Bacterial and virus strains Ad5-SARS CoV2 S University of Iowa Vector Core, custom made N/A SARS-Related Coronavirus 2, Isolate USA-WA1/2020, BEI resources NR-52281 Chemicals, peptides, and recombinant proteins SARS CoV-2 S protein overlapping peptide pools (181 peptides) BEI resources NR-52402 SARS CoV-2 S protein for ELISA Made by the Northwestern University recombinant protein production core using a SARS CoV-2 S protein gene provided by BEI (NR-52394, see above) N/A Critical commercial assays Quick RNA 96 kit (RNA extraction) Zymo Research R1052 Taqman RNA-to-CT step kit ThermoFisher 4392653 Experimental models: Cell lines HEK-293 ATCC CRL-1573 Vero-E6 ATCC CRL-1586 Experimental models: Organisms/strains K18-hACE2 mice Jackson Laboratories 034060 Recombinant DNA Plasmid containing SARS CoV-2 spike (S) gene BEI resources NR-52394 Software and algorithms GraphPad Prism software version 9.1.0 https://www.graphpad.com/ scientific-software/prism/ N/A FlowJo version 10.7.2 https://www.flowjo.com N/A Other KbVL8 tetramer (APC) Provided by the NIH tetramer core at Emory University N/A Forward primer, 50 GACCCCAAAATCAGCGAAAT-30 Integrated DNA Technologies 10006713 (Continued on next page) Cell Reports 42, 112167, March 28, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Reverse primer, 50 TCTGTTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 Probe, 50 TCTGGTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 SARS CoV-2 copy number control BEI resources NR-52358 Live dead dye for flow cytometry Invitrogen L34976 Cytofix/cytoperm (fixing agent for flow cytometry) BD Biosciences 554722 Phosphate buffer saline (PBS) Gibco 14190-144 Golgi Plug BD Biosciences 555029 Golgi Stop BD Biosciences 554724 DMEM Gibco 11965-092 Fetal bovine serum Sigma F0926-500

    Plasmid Preparation:

    Article Title: Pre-existing immunity modulates responses to mRNA boosters.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD8, clone 53-6.7 in PerCPcy5.5 BD Pharmingen Cat# 551162, RRID:AB_394081 aCD44, clone IM7 in Pacific Blue Biolegend Cat# 103020, RRID:AB_493683 aIFNy, clone XMG1.2 in APC BD Pharmingen Cat# 554413, RRID:AB_398551 aCD4, clone RM4-5 FITC eBioscience Cat# 11-0042-82, RRID:AB_464896 Goat a mouse IgG-biotin, clone RM4-5 FITC Invitrogen Cat# SA5-10239, RRID:AB_2810197 Streptavidin (SA)-APC Invitrogen S868 biotin anti-mouse IgG1[a] BD-Pharmingen Cat# 553500, RRID:AB_394885 biotin anti-mouse IgG1[b] BD-Pharmingen Cat# 553533, RRID:AB_394903 anti-SARS-CoV-2 S protein (RBD epitope B), clone SARS2-34 BioXCell Cat# BE0359, RRID:AB_2894778 anti-SARS-CoV-2 S protein (RBD epitope A), clone SARS2-01 BioXCell Cat# BE0357, RRID:AB_2894776 Bacterial and virus strains Ad5-SARS CoV2 S University of Iowa Vector Core, custom made N/A SARS-Related Coronavirus 2, Isolate USA-WA1/2020, BEI resources NR-52281 Chemicals, peptides, and recombinant proteins SARS CoV-2 S protein overlapping peptide pools (181 peptides) BEI resources NR-52402 SARS CoV-2 S protein for ELISA Made by the Northwestern University recombinant protein production core using a SARS CoV-2 S protein gene provided by BEI (NR-52394, see above) N/A Critical commercial assays Quick RNA 96 kit (RNA extraction) Zymo Research R1052 Taqman RNA-to-CT step kit ThermoFisher 4392653 Experimental models: Cell lines HEK-293 ATCC CRL-1573 Vero-E6 ATCC CRL-1586 Experimental models: Organisms/strains K18-hACE2 mice Jackson Laboratories 034060 Recombinant DNA Plasmid containing SARS CoV-2 spike (S) gene BEI resources NR-52394 Software and algorithms GraphPad Prism software version 9.1.0 https://www.graphpad.com/ scientific-software/prism/ N/A FlowJo version 10.7.2 https://www.flowjo.com N/A Other KbVL8 tetramer (APC) Provided by the NIH tetramer core at Emory University N/A Forward primer, 50 GACCCCAAAATCAGCGAAAT-30 Integrated DNA Technologies 10006713 (Continued on next page) Cell Reports 42, 112167, March 28, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Reverse primer, 50 TCTGTTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 Probe, 50 TCTGGTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 SARS CoV-2 copy number control BEI resources NR-52358 Live dead dye for flow cytometry Invitrogen L34976 Cytofix/cytoperm (fixing agent for flow cytometry) BD Biosciences 554722 Phosphate buffer saline (PBS) Gibco 14190-144 Golgi Plug BD Biosciences 555029 Golgi Stop BD Biosciences 554724 DMEM Gibco 11965-092 Fetal bovine serum Sigma F0926-500

    Software:

    Article Title: Pre-existing immunity modulates responses to mRNA boosters.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD8, clone 53-6.7 in PerCPcy5.5 BD Pharmingen Cat# 551162, RRID:AB_394081 aCD44, clone IM7 in Pacific Blue Biolegend Cat# 103020, RRID:AB_493683 aIFNy, clone XMG1.2 in APC BD Pharmingen Cat# 554413, RRID:AB_398551 aCD4, clone RM4-5 FITC eBioscience Cat# 11-0042-82, RRID:AB_464896 Goat a mouse IgG-biotin, clone RM4-5 FITC Invitrogen Cat# SA5-10239, RRID:AB_2810197 Streptavidin (SA)-APC Invitrogen S868 biotin anti-mouse IgG1[a] BD-Pharmingen Cat# 553500, RRID:AB_394885 biotin anti-mouse IgG1[b] BD-Pharmingen Cat# 553533, RRID:AB_394903 anti-SARS-CoV-2 S protein (RBD epitope B), clone SARS2-34 BioXCell Cat# BE0359, RRID:AB_2894778 anti-SARS-CoV-2 S protein (RBD epitope A), clone SARS2-01 BioXCell Cat# BE0357, RRID:AB_2894776 Bacterial and virus strains Ad5-SARS CoV2 S University of Iowa Vector Core, custom made N/A SARS-Related Coronavirus 2, Isolate USA-WA1/2020, BEI resources NR-52281 Chemicals, peptides, and recombinant proteins SARS CoV-2 S protein overlapping peptide pools (181 peptides) BEI resources NR-52402 SARS CoV-2 S protein for ELISA Made by the Northwestern University recombinant protein production core using a SARS CoV-2 S protein gene provided by BEI (NR-52394, see above) N/A Critical commercial assays Quick RNA 96 kit (RNA extraction) Zymo Research R1052 Taqman RNA-to-CT step kit ThermoFisher 4392653 Experimental models: Cell lines HEK-293 ATCC CRL-1573 Vero-E6 ATCC CRL-1586 Experimental models: Organisms/strains K18-hACE2 mice Jackson Laboratories 034060 Recombinant DNA Plasmid containing SARS CoV-2 spike (S) gene BEI resources NR-52394 Software and algorithms GraphPad Prism software version 9.1.0 https://www.graphpad.com/ scientific-software/prism/ N/A FlowJo version 10.7.2 https://www.flowjo.com N/A Other KbVL8 tetramer (APC) Provided by the NIH tetramer core at Emory University N/A Forward primer, 50 GACCCCAAAATCAGCGAAAT-30 Integrated DNA Technologies 10006713 (Continued on next page) Cell Reports 42, 112167, March 28, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Reverse primer, 50 TCTGTTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 Probe, 50 TCTGGTTACTGCCAGTTGAATCTG-30 Integrated DNA Technologies 10006713 SARS CoV-2 copy number control BEI resources NR-52358 Live dead dye for flow cytometry Invitrogen L34976 Cytofix/cytoperm (fixing agent for flow cytometry) BD Biosciences 554722 Phosphate buffer saline (PBS) Gibco 14190-144 Golgi Plug BD Biosciences 555029 Golgi Stop BD Biosciences 554724 DMEM Gibco 11965-092 Fetal bovine serum Sigma F0926-500



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    ATCC cell lines hek 293 atcc crl 1573 vero e6 atcc crl
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